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KMID : 0605619990060010067
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Journal of Korean Society of Biological Psychiatry
1999 Volume.6 No. 1 p.67 ~ p.73
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Screening of Differentially Expressed Genes between PC12 Cells and A123.7 Cells
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1백승연/1Seung Youn Baik
1채영규/2양병환/1Young Gyu Chai/2Byung Hwan Yang
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Abstract
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The cAMP-dependent protein kinase(PKA) is an intracellular enzyme with
serine-threonine kinase activity that plays a key role in cell growth, differentiation, and
apoptosis in eukaryotes. In order to understand the PKA signal transduction pathway
regulating cell life cycle and identify its role, we focused on the characterization of
up-/down-regulated genes by PKA using the differential display polymerase chain
reaction. Seven differentially expressed sequence tags(DEST) have been obtained.
Among these DESTs, 2DESTs were homologous to the sequence of genes from BLAST
search result. KC1-5 DEST that was up-regulated in A123.7 cells was highly
corresponded to mouse apoptosis-related gene(MA-3) or mouse mRNA for topoisomerase
inhibitor suppressed(TIS). MA-3 was induced in various types of apoptosis, specially in
NGF-deprived apoptotic PC12 cells, TIS was down-regulated in the RVC lymphoma
cells incubated with topoisomerase inhibitor that induces DNA strand breakages.
PG1-1DEST that was highly expressed in PC12 cells was corresponded to transposon
Tn103'-end. Tnansposon Tn10 was up-regulated in differentiated myeloblastic ML-1
cells by 12-Ο-tetradecanoylphorbol-13-acetate. This study illuminates that MA-3/TIS
was down-regulated by PKA activity, and transposon Tn10 was up-regulated by it.
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